Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris

Ruth, Claudia; Zuellig, Thomas; Mellitzer, Andrea; Weiss, Roland; Looser, Verena; Kovar, Karin; Glieder, Anton

doi:10.1007/s11693-010-9057-0

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris
Authors:	Ruth, Claudia Zuellig, Thomas Mellitzer, Andrea Weiss, Roland Looser, Verena Kovar, Karin Glieder, Anton
DOI:	10.1007/s11693-010-9057-0
Published in:	Systems and Synthetic Biology
Volume(Issue):	4
Issue:	3
Page(s):	181
Pages to:	191
Issue Date:	29-May-2010
Publisher / Ed. Institution:	Springer
ISSN:	1872-5325
Language:	English
Subjects:	Porcine trypsinogen; Production window; Pichia pastoris; AOX1 promoter variants
Subject (DDC):	660.6: Biotechnology
Abstract:	Natural tools for recombinant protein production show technological limitations. Available natural promoters for gene expression in Pichia pastoris are either constitutive, weak or require the use of undesirable substances or procedures for induction. Here we show the application of deletion variants based on the well known methanol inducible AOX1 promoter and small synthetic promoters, where cis-acting elements were fused to core promoter fragments. They enable differently regulated target protein expression and at the same time to replace methanol induction by a glucose or glycerol feeding strategy. Trypsinogen, the precursor of the serine protease trypsin, was expressed using these different promoters. Depending on the applied promoter the production window (i.e. the time of increasing product concentration) changed significantly. In fedbatch processes trypsinogen yields before induction with methanol were up to 10 times higher if variants of the AOX1 promoter were applied. In addition, the starting point of autoproteolytic product degradation can be predetermined by the promoter choice.
URI:	https://digitalcollection.zhaw.ch/handle/11475/11466 http://europepmc.org/articles/PMC2955199?pdf=render
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Ruth, C., Zuellig, T., Mellitzer, A., Weiss, R., Looser, V., Kovar, K., & Glieder, A. (2010). Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris. Systems and Synthetic Biology, 4(3), 181–191. https://doi.org/10.1007/s11693-010-9057-0

Ruth, C. et al. (2010) ‘Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris’, Systems and Synthetic Biology, 4(3), pp. 181–191. Available at: https://doi.org/10.1007/s11693-010-9057-0.

C. Ruth et al., “Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris,” Systems and Synthetic Biology, vol. 4, no. 3, pp. 181–191, May 2010, doi: 10.1007/s11693-010-9057-0.

RUTH, Claudia, Thomas ZUELLIG, Andrea MELLITZER, Roland WEISS, Verena LOOSER, Karin KOVAR und Anton GLIEDER, 2010. Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris. Systems and Synthetic Biology [online]. 29 Mai 2010. Bd. 4, Nr. 3, S. 181–191. DOI 10.1007/s11693-010-9057-0. Verfügbar unter: http://europepmc.org/articles/PMC2955199?pdf=render

Ruth, Claudia, Thomas Zuellig, Andrea Mellitzer, Roland Weiss, Verena Looser, Karin Kovar, and Anton Glieder. 2010. “Variable Production Windows for Porcine Trypsinogen Employing Synthetic Inducible Promoter Variants in Pichia Pastoris.” Systems and Synthetic Biology 4 (3): 181–91. https://doi.org/10.1007/s11693-010-9057-0.

Ruth, Claudia, et al. “Variable Production Windows for Porcine Trypsinogen Employing Synthetic Inducible Promoter Variants in Pichia Pastoris.” Systems and Synthetic Biology, vol. 4, no. 3, May 2010, pp. 181–91, https://doi.org/10.1007/s11693-010-9057-0.