A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes

Rohrer, Jack; Schweizer, Anja; Johnson, Karl F.; Kornfeld, Stuart

doi:10.1083/jcb.130.6.1297

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes
Authors:	Rohrer, Jack Schweizer, Anja Johnson, Karl F. Kornfeld, Stuart
DOI:	10.1083/jcb.130.6.1297
Published in:	Journal of Cell Biology
Volume(Issue):	130
Issue:	6
Page(s):	1297
Pages to:	1306
Issue Date:	15-Sep-1995
Publisher / Ed. Institution:	Rockefeller University Press
ISSN:	0021-9525
Language:	English
Subject (DDC):	572: Biochemistry
Abstract:	The bovine cation-dependent mannose 6-phosphate receptor (CD-MPR) is a type 1 transmembrane protein that cycles between the trans-Golgi network, endosomes, and the plasma membrane. When the terminal 40 residues were deleted from the 67-amino acid cytoplasmic tail of the CD-MPR, the half-life of the receptor was drastically decreased and the mutant receptor was recovered in lysosomes. Analysis of additional cytoplasmic tail truncation mutants and alanine-scanning mutants implicated amino acids 34-39 as being critical for avoidance of lysosomal degradation. The cytoplasmic tail of the CD-MPR was partially effective in preventing the lysosomal membrane protein Lamp1 from entering lysosomes. Complete exclusion required both the CD-MPR cytoplasmic tail and transmembrane domain. The transmembrane domain alone had just a minor effect on the distribution of Lamp1. These findings indicate that the cytoplasmic tail of the CD-MPR contains a signal that prevents the receptor from trafficking to lysosomes. The transmembrane domain of the CD-MPR also contributes to this function.
URI:	https://digitalcollection.zhaw.ch/handle/11475/6831
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Appears in collections:	Publikationen Life Sciences und Facility Management

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Rohrer, J., Schweizer, A., Johnson, K. F., & Kornfeld, S. (1995). A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes. Journal of Cell Biology, 130(6), 1297–1306. https://doi.org/10.1083/jcb.130.6.1297

Rohrer, J. et al. (1995) ‘A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes’, Journal of Cell Biology, 130(6), pp. 1297–1306. Available at: https://doi.org/10.1083/jcb.130.6.1297.

J. Rohrer, A. Schweizer, K. F. Johnson, and S. Kornfeld, “A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes,” Journal of Cell Biology, vol. 130, no. 6, pp. 1297–1306, Sep. 1995, doi: 10.1083/jcb.130.6.1297.

ROHRER, Jack, Anja SCHWEIZER, Karl F. JOHNSON und Stuart KORNFELD, 1995. A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes. Journal of Cell Biology. 15 September 1995. Bd. 130, Nr. 6, S. 1297–1306. DOI 10.1083/jcb.130.6.1297

Rohrer, Jack, Anja Schweizer, Karl F. Johnson, and Stuart Kornfeld. 1995. “A Determinant in the Cytoplasmic Tail of the Cation-Dependent Mannose 6-Phosphate Receptor Prevents Trafficking to Lysosomes.” Journal of Cell Biology 130 (6): 1297–1306. https://doi.org/10.1083/jcb.130.6.1297.

Rohrer, Jack, et al. “A Determinant in the Cytoplasmic Tail of the Cation-Dependent Mannose 6-Phosphate Receptor Prevents Trafficking to Lysosomes.” Journal of Cell Biology, vol. 130, no. 6, Sept. 1995, pp. 1297–306, https://doi.org/10.1083/jcb.130.6.1297.