| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast |
| Authors: | Rohrer, Jack Bénédetti, Hélène Zanolari, Bettina Riezman, Howard |
| DOI: | 10.1091/mbc.4.5.511 |
| Published in: | Molecular Biology of the Cell |
| Volume(Issue): | 4 |
| Issue: | 5 |
| Page(s): | 511 |
| Pages to: | 521 |
| Issue Date: | May-1993 |
| Publisher / Ed. Institution: | American Society for Cell Biology |
| ISSN: | 1939-4586 1059-1524 |
| Language: | English |
| Subject (DDC): | 571: Physiology and related subjects 572: Biochemistry |
| Abstract: | The Saccharomyces cerevisiae alpha-pheromone receptor, a polytopic, G protein-coupled, membrane protein, is internalized after binding of alpha-factor. Mutational analysis suggested that the first 39 residues of the receptor's cytoplasmic tail carries sufficient information for internalization. A point mutation in one of these 39 residues, K337 to R337, renders the receptor nonfunctional for endocytosis. Other residues, D335 and S338, contribute to the efficiency of internalization. When the sequence DAKSS is added onto a severely truncated receptor, endocytosis of the receptor is restored, showing that this sequence functions to mediate or to signal interaction with the endocytic machinery. Analysis of pheromone response and recovery in strains expressing mutant receptors suggests that receptor internalization is not important for response but contributes to recovery from pheromone. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/6798 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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Rohrer, J., Bénédetti, H., Zanolari, B., & Riezman, H. (1993). Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast. Molecular Biology of the Cell, 4(5), 511–521. https://doi.org/10.1091/mbc.4.5.511
Rohrer, J. et al. (1993) ‘Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast’, Molecular Biology of the Cell, 4(5), pp. 511–521. Available at: https://doi.org/10.1091/mbc.4.5.511.
J. Rohrer, H. Bénédetti, B. Zanolari, and H. Riezman, “Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast,” Molecular Biology of the Cell, vol. 4, no. 5, pp. 511–521, May 1993, doi: 10.1091/mbc.4.5.511.
ROHRER, Jack, Hélène BÉNÉDETTI, Bettina ZANOLARI und Howard RIEZMAN, 1993. Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast. Molecular Biology of the Cell. Mai 1993. Bd. 4, Nr. 5, S. 511–521. DOI 10.1091/mbc.4.5.511
Rohrer, Jack, Hélène Bénédetti, Bettina Zanolari, and Howard Riezman. 1993. “Identification of a Novel Sequence Mediating Regulated Endocytosis of the G Protein-Coupled Alpha-Pheromone Receptor in Yeast.” Molecular Biology of the Cell 4 (5): 511–21. https://doi.org/10.1091/mbc.4.5.511.
Rohrer, Jack, et al. “Identification of a Novel Sequence Mediating Regulated Endocytosis of the G Protein-Coupled Alpha-Pheromone Receptor in Yeast.” Molecular Biology of the Cell, vol. 4, no. 5, May 1993, pp. 511–21, https://doi.org/10.1091/mbc.4.5.511.
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