| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Genomic effects of polyamide/DNA interactions on mRNA expression |
| Autor/-in: | Supekova, Lubica Pezacki, John Paul Su, Andrew I. Loweth, Colin J. Riedl, Rainer Geierstanger, Bernhard Schultz, Peter G. Wemmer, David E. |
| DOI: | 10.1016/S1074-5521(02)00174-6 |
| Erschienen in: | Chemistry & Biology |
| Band(Heft): | 9 |
| Heft: | 7 |
| Seite(n): | 821 |
| Seiten bis: | 827 |
| Erscheinungsdatum: | 2002 |
| Verlag / Hrsg. Institution: | Cell Press |
| ISSN: | 1074-5521 1879-1301 |
| Sprache: | Englisch |
| Schlagwörter: | Gene chip; Gene expression |
| Fachgebiet (DDC): | 572: Biochemie |
| Zusammenfassung: | Here we characterize the biological activity of a hairpin polyamide 1 that inhibits binding of the minor-groove transcription factor LEF-1, constitutively expressed in colon cancers. Genome-wide analysis of mRNA expression in DLD1 colon cancer cells treated with 1 reveals that a limited number of genes are affected; the most significant changes correspond to genes related to cell cycle, signaling, and proteolysis rather than the anticipated WNT signaling pathway. Treated cells display increased doubling time and hypersensitivity to DNA damage that most likely results from downregulation of DNA-damage checkpoint genes, including YWAE (14-3-3ϵ protein) and DDIT3. Promoter analyses on a genomic level revealed numerous potential polyamide binding sites and multiple possible mechanisms for transcriptional antagonism, underscoring the utility of gene expression profiling in understanding the effects of polyamides on transcription at the cellular level. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/2856 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Supekova, L., Pezacki, J. P., Su, A. I., Loweth, C. J., Riedl, R., Geierstanger, B., Schultz, P. G., & Wemmer, D. E. (2002). Genomic effects of polyamide/DNA interactions on mRNA expression. Chemistry & Biology, 9(7), 821–827. https://doi.org/10.1016/S1074-5521(02)00174-6
Supekova, L. et al. (2002) ‘Genomic effects of polyamide/DNA interactions on mRNA expression’, Chemistry & Biology, 9(7), pp. 821–827. Available at: https://doi.org/10.1016/S1074-5521(02)00174-6.
L. Supekova et al., “Genomic effects of polyamide/DNA interactions on mRNA expression,” Chemistry & Biology, vol. 9, no. 7, pp. 821–827, 2002, doi: 10.1016/S1074-5521(02)00174-6.
SUPEKOVA, Lubica, John Paul PEZACKI, Andrew I. SU, Colin J. LOWETH, Rainer RIEDL, Bernhard GEIERSTANGER, Peter G. SCHULTZ und David E. WEMMER, 2002. Genomic effects of polyamide/DNA interactions on mRNA expression. Chemistry & Biology. 2002. Bd. 9, Nr. 7, S. 821–827. DOI 10.1016/S1074-5521(02)00174-6
Supekova, Lubica, John Paul Pezacki, Andrew I. Su, Colin J. Loweth, Rainer Riedl, Bernhard Geierstanger, Peter G. Schultz, and David E. Wemmer. 2002. “Genomic Effects of Polyamide/DNA Interactions on mRNA Expression.” Chemistry & Biology 9 (7): 821–27. https://doi.org/10.1016/S1074-5521(02)00174-6.
Supekova, Lubica, et al. “Genomic Effects of Polyamide/DNA Interactions on mRNA Expression.” Chemistry & Biology, vol. 9, no. 7, 2002, pp. 821–27, https://doi.org/10.1016/S1074-5521(02)00174-6.
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