Fast single-use VLP vaccine productions based on insect cells and the baculovirus expression vector system : influenza as case study

Eibl-Schindler, Regine; Steiger, Nina; Wellnitz, Sabine; Vicente, Tiago; John, Corinne; Eibl, Dieter

doi:10.1007/10_2013_186

Publication type:	Book part
Type of review:	Editorial review
Title:	Fast single-use VLP vaccine productions based on insect cells and the baculovirus expression vector system : influenza as case study
Authors:	Eibl-Schindler, Regine Steiger, Nina Wellnitz, Sabine Vicente, Tiago John, Corinne Eibl, Dieter
DOI:	10.1007/10_2013_186
Published in:	Disposable bioreactors II
Editors of the parent work:	Eibl, Dieter Eibl-Schindler, Regine
Page(s):	99
Pages to:	125
Issue Date:	2014
Series:	Advances in Biochemical Engineering/Biotechnology
Series volume:	138
Publisher / Ed. Institution:	Springer
Publisher / Ed. Institution:	Berlin
ISBN:	978-3-642-45157-7 978-3-642-45158-4
Language:	English
Subjects:	DSP; Influenza; VLP vaccine; Sf-9/BEVS USP; Wave-mixed bioreactor
Subject (DDC):	615: Pharmacology and therapeutics
Abstract:	During the last few years virus like particles (VLPs) have become increasingly interesting for the production of vaccines. This development is explained by their excellent safety profile as well as a significant number of clinical studies showing strong and long-lasting protection. A further reason is the possibility of speeding up VLP vaccine manufacturing by implementing single-use (SU) technology in the case of mammalian and insect-cell-based processes, for which a multitude of SU devices up to middle-volume scale already exist. After briefly introducing the vaccine types and expression systems currently in use, this chapter turns to VLP vaccines and the insect cell/baculovirus expression vector system (IC/BEVS). Based on the main process characteristics and typical process flow of IC/BEVS-based VLP vaccine productions, suitable SU devices and their implementation are addressed. We subsequently report on the successful development of a fast, scalable benchtop production process generating a four-protein component influenza A H1N1 VLP vaccine candidate. This process is based on Spodoptera frugiperda (Sf)-9 cells and combines Redbiotec’s rePAXTM technology with obtainable SU devices for upstream (USP) and downstream processing (DSP).
URI:	https://digitalcollection.zhaw.ch/handle/11475/2254
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

There are no files associated with this item.

Show full item record

Eibl-Schindler, R., Steiger, N., Wellnitz, S., Vicente, T., John, C., & Eibl, D. (2014). Fast single-use VLP vaccine productions based on insect cells and the baculovirus expression vector system : influenza as case study. In D. Eibl & R. Eibl-Schindler (Eds.), Disposable bioreactors II (pp. 99–125). Springer. https://doi.org/10.1007/10_2013_186

Eibl-Schindler, R. et al. (2014) ‘Fast single-use VLP vaccine productions based on insect cells and the baculovirus expression vector system : influenza as case study’, in D. Eibl and R. Eibl-Schindler (eds) Disposable bioreactors II. Berlin: Springer, pp. 99–125. Available at: https://doi.org/10.1007/10_2013_186.

R. Eibl-Schindler, N. Steiger, S. Wellnitz, T. Vicente, C. John, and D. Eibl, “Fast single-use VLP vaccine productions based on insect cells and the baculovirus expression vector system : influenza as case study,” in Disposable bioreactors II, D. Eibl and R. Eibl-Schindler, Eds. Berlin: Springer, 2014, pp. 99–125. doi: 10.1007/10_2013_186.

EIBL-SCHINDLER, Regine, Nina STEIGER, Sabine WELLNITZ, Tiago VICENTE, Corinne JOHN und Dieter EIBL, 2014. Fast single-use VLP vaccine productions based on insect cells and the baculovirus expression vector system : influenza as case study. In: Dieter EIBL und Regine EIBL-SCHINDLER (Hrsg.), Disposable bioreactors II. Berlin: Springer. S. 99–125. ISBN 978-3-642-45157-7

Eibl-Schindler, Regine, Nina Steiger, Sabine Wellnitz, Tiago Vicente, Corinne John, and Dieter Eibl. 2014. “Fast Single-Use VLP Vaccine Productions Based on Insect Cells and the Baculovirus Expression Vector System : Influenza as Case Study.” In Disposable Bioreactors II, edited by Dieter Eibl and Regine Eibl-Schindler, 99–125. Berlin: Springer. https://doi.org/10.1007/10_2013_186.

Eibl-Schindler, Regine, et al. “Fast Single-Use VLP Vaccine Productions Based on Insect Cells and the Baculovirus Expression Vector System : Influenza as Case Study.” Disposable Bioreactors II, edited by Dieter Eibl and Regine Eibl-Schindler, Springer, 2014, pp. 99–125, https://doi.org/10.1007/10_2013_186.