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dc.contributor.authorKremer, Antje-
dc.contributor.authorWussmann, Maximiliane-
dc.contributor.authorHerrmann, Marietta-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorWalles, Heike-
dc.date.accessioned2021-03-26T15:17:24Z-
dc.date.available2021-03-26T15:17:24Z-
dc.date.issued2019-12-17-
dc.identifier.issn1386-0291de_CH
dc.identifier.issn1875-8622de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/22186-
dc.description.abstractBackground: Prolyl hydroxylase inhibitors (PHIs) are promising compounds to promote angiogenesis by stabilizing hypoxia-inducible factor-1α (HIF-1α), a master regulator of angiogenesis. Increased HIF-1α presence induces expression of proangiogenic genes such as vascular endothelial growth factor (VEGF). Objective:We investigated the pharmacological induction of hypoxia via the PHI ciclopirox olamine (CPX) as angiogenesis strategy on human dermal microvascular endothelial cell (hd-mvEC) spheroids directly and indirectly via activating human mesenchymal stem cells (hMSCs). Methods: HMSCs were isolated from bone marrow and hd-mvECs from foreskin biopsies. MSC-conditioned medium after CPX stimulation (MSC-CM CPX) was analyzed by VEGF ELISA and Proteome Profiler™ Human Angiogenesis Array. Direct stimulation with CPX and indirect stimulation via MSC-CM CPX were compared in sprouting assays of hd-mvEC spheroids. Results: Direct stimulation with CPX significantly increased sprouting of hd-mvEC spheroids. MSC-CM CPX also induced sprouting from hd-mvEC spheroids, which was mediated by angiogenic VEGF and other proangiogenic factors that had been produced by stimulated hMSCs. Conclusions: The stimulation with CPX increased the proangiogenic response of hd-mvECs and hMSCs. The direct stimulation of hd-mvECs with CPX has the potential to replace external VEGF supplementation. Thus, CPX can induce angiogenesis in ECs even in the absence of auxiliary cells demonstrating a promising proangiogenic approach.de_CH
dc.language.isoende_CH
dc.publisherIOS Pressde_CH
dc.relation.ispartofClinical Hemorheology and Microcirculationde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectHIF-1αde_CH
dc.subjectAngiogenesisde_CH
dc.subjectCiclopirox olaminede_CH
dc.subjectEndothelial cellsde_CH
dc.subjectMesenchymal stem cellsde_CH
dc.subjectProlyl hydroxylase inhibitorde_CH
dc.subjectSproutingde_CH
dc.subjectVascularizationde_CH
dc.subjectCiclopiroxde_CH
dc.subjectGene expressionde_CH
dc.subjectHumande_CH
dc.subjectPathologic neovascularizationde_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleCiclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cellsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3233/CH-190559de_CH
dc.identifier.pmid31006674de_CH
zhaw.funding.euNode_CH
zhaw.issue2de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end328de_CH
zhaw.pages.start317de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume73de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Kremer, A., Wussmann, M., Herrmann, M., Raghunath, M., & Walles, H. (2019). Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells. Clinical Hemorheology and Microcirculation, 73(2), 317–328. https://doi.org/10.3233/CH-190559
Kremer, A. et al. (2019) ‘Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells’, Clinical Hemorheology and Microcirculation, 73(2), pp. 317–328. Available at: https://doi.org/10.3233/CH-190559.
A. Kremer, M. Wussmann, M. Herrmann, M. Raghunath, and H. Walles, “Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells,” Clinical Hemorheology and Microcirculation, vol. 73, no. 2, pp. 317–328, Dec. 2019, doi: 10.3233/CH-190559.
KREMER, Antje, Maximiliane WUSSMANN, Marietta HERRMANN, Michael RAGHUNATH und Heike WALLES, 2019. Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells. Clinical Hemorheology and Microcirculation. 17 Dezember 2019. Bd. 73, Nr. 2, S. 317–328. DOI 10.3233/CH-190559
Kremer, Antje, Maximiliane Wussmann, Marietta Herrmann, Michael Raghunath, and Heike Walles. 2019. “Ciclopirox Olamine Promotes the Angiogenic Response of Endothelial Cells and Mesenchymal Stem Cells.” Clinical Hemorheology and Microcirculation 73 (2): 317–28. https://doi.org/10.3233/CH-190559.
Kremer, Antje, et al. “Ciclopirox Olamine Promotes the Angiogenic Response of Endothelial Cells and Mesenchymal Stem Cells.” Clinical Hemorheology and Microcirculation, vol. 73, no. 2, Dec. 2019, pp. 317–28, https://doi.org/10.3233/CH-190559.


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