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https://doi.org/10.21256/zhaw-21921
Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
Art der Begutachtung: | Peer review (Publikation) |
Titel: | Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells |
Autor/-in: | Panella, Stefano Muoio, Francesco Jossen, Valentin Harder, Yves Eibl-Schindler, Regine Tallone, Tiziano |
et. al: | No |
DOI: | 10.3390/cells10020466 10.21256/zhaw-21921 |
Erschienen in: | Cells |
Band(Heft): | 10 |
Heft: | 2 |
Seite(n): | 466 |
Erscheinungsdatum: | 2021 |
Verlag / Hrsg. Institution: | MDPI |
ISSN: | 2073-4409 |
Sprache: | Englisch |
Schlagwörter: | UrSuppe; Defined cell culture; Xeno- and serum-free cell culture; Adipogenic differentiated adipose-derived stromal cell; White and beige/brown adipocyte; Platelet lysate |
Fachgebiet (DDC): | 610.28: Biomedizin, Biomedizinische Technik |
Zusammenfassung: | Adipose tissue is an abundant source of stem cells. However, liposuction cannot yield cell quantities sufficient for direct applications in regenerative medicine. Therefore, the development of GMP-compliant ex vivo expansion protocols is required to ensure the production of a “cell drug” that is safe, reproducible, and cost-effective. Thus, we developed our own basal defined xeno- and serum-free cell culture medium (UrSuppe), specifically formulated to grow human adipose stem cells (hASCs). With this medium, we can directly culture the stromal vascular fraction (SVF) cells in defined cell culture conditions to obtain hASCs. Cells proliferate while remaining undifferentiated, as shown by Flow Cytometry (FACS), Quantitative Reverse Transcription PCR (RT-qPCR) assays, and their secretion products. Using the UrSuppe cell culture medium, maximum cell densities between 0.51 and 0.80 × 105 cells/cm2 (=2.55–4.00 × 105 cells/mL) were obtained. As the expansion of hASCs represents only the first step in a cell therapeutic protocol or further basic research studies, we formulated two chemically defined media to differentiate the expanded hASCs in white or beige/brown adipocytes. These new media could help translate research projects into the clinical application of hASCs and study ex vivo the biology in healthy and dysfunctional states of adipocytes and their precursors. Following the cell culture system developers’ practice and obvious reasons related to the formulas’ patentability, the defined media’s composition will not be disclosed in this study. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/21921 |
Volltext Version: | Publizierte Version |
Lizenz (gemäss Verlagsvertrag): | CC BY 4.0: Namensnennung 4.0 International |
Departement: | Life Sciences und Facility Management |
Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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2021_Panella-etal_Medium-human-adipose-stem-cells.pdf | 10.17 MB | Adobe PDF | Öffnen/Anzeigen |
Zur Langanzeige
Panella, S., Muoio, F., Jossen, V., Harder, Y., Eibl-Schindler, R., & Tallone, T. (2021). Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells. Cells, 10(2), 466. https://doi.org/10.3390/cells10020466
Panella, S. et al. (2021) ‘Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells’, Cells, 10(2), p. 466. Available at: https://doi.org/10.3390/cells10020466.
S. Panella, F. Muoio, V. Jossen, Y. Harder, R. Eibl-Schindler, and T. Tallone, “Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells,” Cells, vol. 10, no. 2, p. 466, 2021, doi: 10.3390/cells10020466.
PANELLA, Stefano, Francesco MUOIO, Valentin JOSSEN, Yves HARDER, Regine EIBL-SCHINDLER und Tiziano TALLONE, 2021. Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells. Cells. 2021. Bd. 10, Nr. 2, S. 466. DOI 10.3390/cells10020466
Panella, Stefano, Francesco Muoio, Valentin Jossen, Yves Harder, Regine Eibl-Schindler, and Tiziano Tallone. 2021. “Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells.” Cells 10 (2): 466. https://doi.org/10.3390/cells10020466.
Panella, Stefano, et al. “Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells.” Cells, vol. 10, no. 2, 2021, p. 466, https://doi.org/10.3390/cells10020466.
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