Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European‐sourced comfrey (Symphytum officinale L.) root samples

Trifan, Adriana; Wolfram, Evelyn; Esslinger, Nils; Grubelnik, Andreas; Skalicka‐Woźniak, Krystyna; Minceva, Mirjana; Luca, Simon Vlad

doi:10.1002/pca.2996

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-20652

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European‐sourced comfrey (Symphytum officinale L.) root samples
Authors:	Trifan, Adriana Wolfram, Evelyn Esslinger, Nils Grubelnik, Andreas Skalicka‐Woźniak, Krystyna Minceva, Mirjana Luca, Simon Vlad
et. al:	No
DOI:	10.1002/pca.2996 10.21256/zhaw-20652
Published in:	Phytochemical Analysis
Volume(Issue):	32
Issue:	4
Page(s):	482
Pages to:	494
Issue Date:	2020
Publisher / Ed. Institution:	Wiley
ISSN:	0958-0344 1099-1565
Language:	English
Subjects:	Symphytum officinale L; High-resolution mass spectrometry; Phenolic marker; Pyrrolizidine alkaloid; Rosmarinic acid
Subject (DDC):	572: Biochemistry 615: Pharmacology and therapeutics
Abstract:	Introduction: Symphytum officinale L. (comfrey, Boraginaceae) is a cultivated or spontaneously growing medicinal plant that is traditionally used for the treatment of bone fractures, hematomas, muscle pains and joint pains. A wide range of topical preparations and dried roots for ex tempore applications are marketed in European drug stores or pharmacies. Objective: The aim of this study was to perform the qualitative and quantitative analysis of pyrrolizidine alkaloids (PAs) and phenolic compounds in the hydroethanolic extracts of 16 commercial comfrey root batches purchased from 12 different European countries. Methods: Liquid chromatography hyphenated with high-resolution tandem mass spectrometry (LC-HRMS/MS) was used for the profiling of PAs and phenolic compounds, whereas LC-MS/MS and liquid chromatography with diode array detection (LC-DAD) were used for their quantification. Results: 20 PAs (i.e. intermedine, lycopsamine, acetylintermedine, acetyllycopsamine, symphytine, symphytine-N-oxide), 17 phenolic compounds (i.e. caffeic and rosmarinic acids, rabdosiin, globoidnan A, globoidnan B) and 9 nonphenolic compounds (sugars, organic and fatty acids) were fully or partly annotated in the analysed samples. In addition, the quantitative analyses revealed that globoidnan B, rabdosiin and globoidnan A are new phenolic markers that can be used together with rosmarinic acid and PAs for the quality control of commercial comfrey root batches. Conclusions: This study brings new insights into the phytochemical complexity of S. officinale, revealing not only numerous toxic PAs, but also a significant number of valuable phenolic compounds that could contribute to the bioactivities of comfreybased preparations
URI:	https://digitalcollection.zhaw.ch/handle/11475/20652
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Trifan, A., Wolfram, E., Esslinger, N., Grubelnik, A., Skalicka‐Woźniak, K., Minceva, M., & Luca, S. V. (2020). Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European‐sourced comfrey (Symphytum officinale L.) root samples. Phytochemical Analysis, 32(4), 482–494. https://doi.org/10.1002/pca.2996

Trifan, A. et al. (2020) ‘Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European‐sourced comfrey (Symphytum officinale L.) root samples’, Phytochemical Analysis, 32(4), pp. 482–494. Available at: https://doi.org/10.1002/pca.2996.

A. Trifan et al., “Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European‐sourced comfrey (Symphytum officinale L.) root samples,” Phytochemical Analysis, vol. 32, no. 4, pp. 482–494, 2020, doi: 10.1002/pca.2996.

TRIFAN, Adriana, Evelyn WOLFRAM, Nils ESSLINGER, Andreas GRUBELNIK, Krystyna SKALICKA‐WOŹNIAK, Mirjana MINCEVA und Simon Vlad LUCA, 2020. Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European‐sourced comfrey (Symphytum officinale L.) root samples. Phytochemical Analysis. 2020. Bd. 32, Nr. 4, S. 482–494. DOI 10.1002/pca.2996

Trifan, Adriana, Evelyn Wolfram, Nils Esslinger, Andreas Grubelnik, Krystyna Skalicka‐Woźniak, Mirjana Minceva, and Simon Vlad Luca. 2020. “Globoidnan A, Rabdosiin and Globoidnan B as New Phenolic Markers in European‐Sourced Comfrey (Symphytum Officinale L.) Root Samples.” Phytochemical Analysis 32 (4): 482–94. https://doi.org/10.1002/pca.2996.

Trifan, Adriana, et al. “Globoidnan A, Rabdosiin and Globoidnan B as New Phenolic Markers in European‐Sourced Comfrey (Symphytum Officinale L.) Root Samples.” Phytochemical Analysis, vol. 32, no. 4, 2020, pp. 482–94, https://doi.org/10.1002/pca.2996.