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https://doi.org/10.21256/zhaw-20295| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Characterization and direct molecular discrimination of rpsL mutations leading to high streptomycin resistance in Erwinia amylovora |
| Authors: | Escursell, Mireia Marcé Roschi, Alexia Smits, Theo Rezzonico, Fabio |
| et. al: | No |
| DOI: | 10.1007/s42161-020-00600-8 10.21256/zhaw-20295 |
| Published in: | Journal of Plant Pathology |
| Volume(Issue): | 103 |
| Issue: | Suppl 1 |
| Page(s): | 99 |
| Pages to: | 108 |
| Issue Date: | 16-Jul-2020 |
| Publisher / Ed. Institution: | Springer |
| ISSN: | 1125-4653 2239-7264 |
| Language: | English |
| Subjects: | Streptomycin resistance; Fire blight; SNaPshot; Mutation |
| Subject (DDC): | 570: Biology |
| Abstract: | Erwinia amylovora can rapidly become highly resistant to applications of the antibiotic streptomycin through a single nucleotide mutation in the rpsL gene, which causes an amino acid substitution that prevents inhibitory binding of streptomycin while preserving the functionality of the ribosome. Several viable mutations are described in the literature, but screenings in the orchards consistently revealed that the one leading to the substitution of lysin by arginine at position 43 (K43R) is by far the most abundant. In this work, we tested the rate of emergence of the various spontaneous mutants, their tolerance to different antibiotic concentrations and their general fitness in competition experiments. Furthermore, we developed a molecular assay based on the SNaPshot™ Multiplex Kit from Thermo Fisher Scientific that allows swift determination of rpsL mutations using a single-base primer extension approach directly from colonies on plate. Or results indicate that the prevalence of variant K43R is largely due to fact that it retains the highest environmental fitness even in absence of antibiotic pressure. Sequence analysis of two resistant colonies that did not respond as expected in the SNaPshot™ assay revealed the existence of two yet undescribed rpsL mutations resulting in conditional-lethal streptomycin-dependent (SmD) phenotypes, which are unable to grow in absence of the antibiotic. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/20295 |
| Fulltext version: | Accepted version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
| Published as part of the ZHAW project: | Schutz von Apfelwälderökosystemen in Zentralasien vor dem bakteriellen Pathogen Erwinia amylovora (Feuerbrand) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2020_Escursell-etal_Characterization-and-direct-molecular-discrimination-of-rpsL-mutations.pdf | Accepted Version | 968.71 kB | Adobe PDF |  View/Open |
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Escursell, M. M., Roschi, A., Smits, T., & Rezzonico, F. (2020). Characterization and direct molecular discrimination of rpsL mutations leading to high streptomycin resistance in Erwinia amylovora. Journal of Plant Pathology, 103(Suppl 1), 99–108. https://doi.org/10.1007/s42161-020-00600-8
Escursell, M.M. et al. (2020) ‘Characterization and direct molecular discrimination of rpsL mutations leading to high streptomycin resistance in Erwinia amylovora’, Journal of Plant Pathology, 103(Suppl 1), pp. 99–108. Available at: https://doi.org/10.1007/s42161-020-00600-8.
M. M. Escursell, A. Roschi, T. Smits, and F. Rezzonico, “Characterization and direct molecular discrimination of rpsL mutations leading to high streptomycin resistance in Erwinia amylovora,” Journal of Plant Pathology, vol. 103, no. Suppl 1, pp. 99–108, Jul. 2020, doi: 10.1007/s42161-020-00600-8.
ESCURSELL, Mireia Marcé, Alexia ROSCHI, Theo SMITS und Fabio REZZONICO, 2020. Characterization and direct molecular discrimination of rpsL mutations leading to high streptomycin resistance in Erwinia amylovora. Journal of Plant Pathology. 16 Juli 2020. Bd. 103, Nr. Suppl 1, S. 99–108. DOI 10.1007/s42161-020-00600-8
Escursell, Mireia Marcé, Alexia Roschi, Theo Smits, and Fabio Rezzonico. 2020. “Characterization and Direct Molecular Discrimination of rpsL Mutations Leading to High Streptomycin Resistance in Erwinia Amylovora.” Journal of Plant Pathology 103 (Suppl 1): 99–108. https://doi.org/10.1007/s42161-020-00600-8.
Escursell, Mireia Marcé, et al. “Characterization and Direct Molecular Discrimination of rpsL Mutations Leading to High Streptomycin Resistance in Erwinia Amylovora.” Journal of Plant Pathology, vol. 103, no. Suppl 1, July 2020, pp. 99–108, https://doi.org/10.1007/s42161-020-00600-8.
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