Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro

Nytko, Katarzina J.; Thumser-Henner, Pauline; Weyland, Mathias S.; Scheidegger, Stephan; Rohrer Bley, Carla

doi:10.1371/journal.pone.0216744

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-19597

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro
Authors:	Nytko, Katarzina J. Thumser-Henner, Pauline Weyland, Mathias S. Scheidegger, Stephan Rohrer Bley, Carla
et. al:	No
DOI:	10.1371/journal.pone.0216744 10.21256/zhaw-19597
Published in:	PLOS ONE
Volume(Issue):	14
Issue:	5
Page(s):	e0216744
Issue Date:	2019
Publisher / Ed. Institution:	Public Library of Science
ISSN:	1932-6203
Language:	English
Subjects:	Hyperthermia; Radiation therapy
Subject (DDC):	615: Pharmacology and therapeutics
Abstract:	Objective: Aims were to investigate sensitivity of various human and canine cancer cell lines to hyperthermia and the influence of particular treatment conditions, and to analyze the DNA-damage response and mode of cell death in cell line radiosensitized by hyperthermia. Additionally, we were interested in the involvement of HSP70 in radiosensitization. Methods: Radiosensitization by hyperthermia was determined in a panel of human and canine cancer cell lines using clonogenic cell survival assay, as well as levels of heat shock proteins (HSPs) using immunoblotting. The influence of the hyperthermia-radiotherapy time gap, different temperatures and the order of treatments on clonogenicity of hyperthermia-sensitive A549 cells was investigated. Additionally, DNA damage and cell death were assessed by Comet assay and an apoptosis/necrosis assay. Further we induced transient knockdown in A549 cells to test HSP70’s involvement in radiosensitization. Results: Out of eight cell lines tested, only two (A549 and Abrams) showed significant decrease in clonogenic cell survival when pre-treated with hyperthermia at 42˚C. Strong induction of HSP70 upon thermoradiotherapy (HT-RT) treatment was found in all cell lines. Transient knockdown of HSP70 in A549 cells did not result in decrease of clonogenic cell survival in response to HT-RT. Conclusion: Tumor cell-type, temperature and order of treatment play an important role in radiosensitization by hyperthermia. However, hyperthermia has limited potency to radiosensitize canine cancer cells grown in a 2D cell culture setting presented here. DNA damage and apoptosis/necrosis did not increase upon combined treatment and cytosolic levels of HSP70 appear not to play critical role in the radiosensitization of A549 cells.
URI:	https://digitalcollection.zhaw.ch/handle/11475/19597
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	School of Engineering
Organisational Unit:	Institute of Applied Mathematics and Physics (IAMP)
Published as part of the ZHAW project:	Dynamic Thermal Dosimetric Concept for Evaluating Synergistic Effect of Combined Hyperthermia-Radiotherapy (RT-HT)
Appears in collections:	Publikationen School of Engineering

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Nytko, K. J., Thumser-Henner, P., Weyland, M. S., Scheidegger, S., & Rohrer Bley, C. (2019). Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro. Plos One, 14(5), e0216744. https://doi.org/10.1371/journal.pone.0216744

Nytko, K.J. et al. (2019) ‘Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro’, PLOS ONE, 14(5), p. e0216744. Available at: https://doi.org/10.1371/journal.pone.0216744.

K. J. Nytko, P. Thumser-Henner, M. S. Weyland, S. Scheidegger, and C. Rohrer Bley, “Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro,” PLOS ONE, vol. 14, no. 5, p. e0216744, 2019, doi: 10.1371/journal.pone.0216744.

NYTKO, Katarzina J., Pauline THUMSER-HENNER, Mathias S. WEYLAND, Stephan SCHEIDEGGER und Carla ROHRER BLEY, 2019. Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro. PLOS ONE. 2019. Bd. 14, Nr. 5, S. e0216744. DOI 10.1371/journal.pone.0216744

Nytko, Katarzina J., Pauline Thumser-Henner, Mathias S. Weyland, Stephan Scheidegger, and Carla Rohrer Bley. 2019. “Cell Line-Specific Efficacy of Thermoradiotherapy in Human and Canine Cancer Cells in Vitro.” Plos One 14 (5): e0216744. https://doi.org/10.1371/journal.pone.0216744.

Nytko, Katarzina J., et al. “Cell Line-Specific Efficacy of Thermoradiotherapy in Human and Canine Cancer Cells in Vitro.” Plos One, vol. 14, no. 5, 2019, p. e0216744, https://doi.org/10.1371/journal.pone.0216744.