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https://doi.org/10.21256/zhaw-18121| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Structural and functional diversity inListeriacell wall teichoic acids |
| Authors: | Shen, Yang Boulos, Samy Sumrall, Eric Gerber, Benjamin Julian-Rodero, Alicia Eugster, Marcel R. Fieseler, Lars Nyström, Laura Ebert, Marc-Olivier Loessner, Martin J. |
| et. al: | No |
| DOI: | 10.1074/jbc.M117.813964 10.21256/zhaw-18121 |
| Published in: | Journal of Biological Chemistry |
| Volume(Issue): | 292 |
| Issue: | 43 |
| Page(s): | 17832 |
| Pages to: | 17844 |
| Issue Date: | 2017 |
| Publisher / Ed. Institution: | American Society for Biochemistry and Molecular Biology |
| ISSN: | 0021-9258 1083-351X |
| Language: | English |
| Subjects: | Listeria; Bacteriophage derived endolysin; Carbohydrate structure; Carbohydrate-binding protein; High-performance liquid chromatography (HPLC); Nuclear magnetic resonance (NMR); Surface plasmon resonance (SPR); Teichoic acid; Acetylglucosamine; Cell wall; Listeria; Ribitol; Species specificity; Teichoic acid |
| Subject (DDC): | 572: Biochemistry |
| Abstract: | Wall teichoic acids (WTAs) are the most abundant glycopolymers found on the cell wall of many Gram-positive bacteria, whose diverse surface structures play key roles in multiple biological processes. Despite recent technological advances in glycan analysis, structural elucidation of WTAs remains challenging due to their complex nature. Here, we employed a combination of ultra-performance liquid chromatography-coupled electrospray ionization tandem-MS/MS and NMR to determine the structural complexity of WTAs from Listeria species. We unveiled more than 10 different types of WTA polymers that vary in their linkage and repeating units. Disparity in GlcNAc to ribitol connectivity, as well as variable O-acetylation and glycosylation of GlcNAc contribute to the structural diversity of WTAs. Notably, SPR analysis indicated that constitution of WTA determines the recognition by bacteriophage endolysins. Collectively, these findings provide detailed insight into Listeria cell wall-associated carbohydrates, and will guide further studies on the structure-function relationship of WTAs. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/18121 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Food and Beverage Innovation (ILGI) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 2017_Shen-etal_Structural-functional-diversity-Listeria-cell-WTA.pdf | 3.47 MB | Adobe PDF |  View/Open |
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Shen, Y., Boulos, S., Sumrall, E., Gerber, B., Julian-Rodero, A., Eugster, M. R., Fieseler, L., Nyström, L., Ebert, M.-O., & Loessner, M. J. (2017). Structural and functional diversity inListeriacell wall teichoic acids. Journal of Biological Chemistry, 292(43), 17832–17844. https://doi.org/10.1074/jbc.M117.813964
Shen, Y. et al. (2017) ‘Structural and functional diversity inListeriacell wall teichoic acids’, Journal of Biological Chemistry, 292(43), pp. 17832–17844. Available at: https://doi.org/10.1074/jbc.M117.813964.
Y. Shen et al., “Structural and functional diversity inListeriacell wall teichoic acids,” Journal of Biological Chemistry, vol. 292, no. 43, pp. 17832–17844, 2017, doi: 10.1074/jbc.M117.813964.
SHEN, Yang, Samy BOULOS, Eric SUMRALL, Benjamin GERBER, Alicia JULIAN-RODERO, Marcel R. EUGSTER, Lars FIESELER, Laura NYSTRÖM, Marc-Olivier EBERT und Martin J. LOESSNER, 2017. Structural and functional diversity inListeriacell wall teichoic acids. Journal of Biological Chemistry. 2017. Bd. 292, Nr. 43, S. 17832–17844. DOI 10.1074/jbc.M117.813964
Shen, Yang, Samy Boulos, Eric Sumrall, Benjamin Gerber, Alicia Julian-Rodero, Marcel R. Eugster, Lars Fieseler, Laura Nyström, Marc-Olivier Ebert, and Martin J. Loessner. 2017. “Structural and Functional Diversity inListeriacell Wall Teichoic Acids.” Journal of Biological Chemistry 292 (43): 17832–44. https://doi.org/10.1074/jbc.M117.813964.
Shen, Yang, et al. “Structural and Functional Diversity inListeriacell Wall Teichoic Acids.” Journal of Biological Chemistry, vol. 292, no. 43, 2017, pp. 17832–44, https://doi.org/10.1074/jbc.M117.813964.
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