Orientational imaging of single molecules by annular illumination

Sick, Beate; Hecht, B.; Novotny, L.

doi:10.1103/PhysRevLett.85.4482

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Orientational imaging of single molecules by annular illumination
Authors:	Sick, Beate Hecht, B. Novotny, L.
DOI:	10.1103/PhysRevLett.85.4482
Published in:	Physical Review Letters
Volume(Issue):	85
Issue:	21
Page(s):	4482
Pages to:	4485
Issue Date:	2000
Publisher / Ed. Institution:	American Physical Society
ISSN:	0031-9007 1079-7114
Language:	English
Subjects:	Dipole pattern; Single molecule; Near field optics
Subject (DDC):	530: Physics
Abstract:	The absorption dipole orientation of single fluorescent molecules is determined by mapping the spatial distribution of the squared electric field components in a high-numerical-aperture laser focus. Annular illumination geometry and the vicinity of a plane dielectric /air interface strongly enhance the longitudinal field component and the transverse fields perpendicular to the polarization direction. As a result, all three excitation field components in the focus are of comparable magnitude. The scheme holds promise to monitor rotational diffusion of single molecules in complex environments.
URI:	https://digitalcollection.zhaw.ch/handle/11475/14039
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	School of Engineering
Organisational Unit:	Institute of Data Analysis and Process Design (IDP)
Appears in collections:	Publikationen School of Engineering

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Sick, B., Hecht, B., & Novotny, L. (2000). Orientational imaging of single molecules by annular illumination. Physical Review Letters, 85(21), 4482–4485. https://doi.org/10.1103/PhysRevLett.85.4482

Sick, B., Hecht, B. and Novotny, L. (2000) ‘Orientational imaging of single molecules by annular illumination’, Physical Review Letters, 85(21), pp. 4482–4485. Available at: https://doi.org/10.1103/PhysRevLett.85.4482.

B. Sick, B. Hecht, and L. Novotny, “Orientational imaging of single molecules by annular illumination,” Physical Review Letters, vol. 85, no. 21, pp. 4482–4485, 2000, doi: 10.1103/PhysRevLett.85.4482.

SICK, Beate, B. HECHT und L. NOVOTNY, 2000. Orientational imaging of single molecules by annular illumination. Physical Review Letters. 2000. Bd. 85, Nr. 21, S. 4482–4485. DOI 10.1103/PhysRevLett.85.4482

Sick, Beate, B. Hecht, and L. Novotny. 2000. “Orientational Imaging of Single Molecules by Annular Illumination.” Physical Review Letters 85 (21): 4482–85. https://doi.org/10.1103/PhysRevLett.85.4482.

Sick, Beate, et al. “Orientational Imaging of Single Molecules by Annular Illumination.” Physical Review Letters, vol. 85, no. 21, 2000, pp. 4482–85, https://doi.org/10.1103/PhysRevLett.85.4482.