| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Pharmacologically induced angiogenesis in transgenic zebrafish |
| Autor/-in: | Raghunath, Michael Wong, Yuan Sy Farooq, Muhammad Gee, Ruowen |
| DOI: | 10.1016/j.bbrc.2008.11.127 |
| Erschienen in: | Biochemical and Biophysical Research Communications |
| Band(Heft): | 378 |
| Heft: | 4 |
| Seite(n): | 766 |
| Seiten bis: | 771 |
| Erscheinungsdatum: | 2009 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0006-291X 1090-2104 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 572: Biochemie |
| Zusammenfassung: | The rapid vascularisation of biomaterials and engineered tissue after implantation is a current unmet need. To this end, we explored the pharmacological option of inducing neovascularisation using compounds that inhibit hypoxia-induced factor-1α prolyl hydroxylase. This stabilises hypoxia inducible factor-1α and therefore de-repress the transcription of various angiogenic genes. In the quest for a small vertebrate model allowing for in vivo screening we exposed transgenic zebrafish embryos exhibiting fluorescent blood vessels to hydralazine hydrochloride and 2,4-pyridine dicarboxylic acid from 6 hpf to 72 hpf by immersion. Live observation of embryos revealed that the substances induced formation of ectopic blood vessels in the subintestinal vessel basket. We confirmed the HIF-stabilising effects biochemically in human fibroblasts and with an in vitro angiogenesis fibroblast/HUVEC co-culture model. Cross-inhibition of collagen prolyl hydroxylase was confirmed by reduced collagen secretion by fibroblasts and reduced collagen content of zebrafish embryos. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12206 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Raghunath, M., Wong, Y. S., Farooq, M., & Gee, R. (2009). Pharmacologically induced angiogenesis in transgenic zebrafish. Biochemical and Biophysical Research Communications, 378(4), 766–771. https://doi.org/10.1016/j.bbrc.2008.11.127
Raghunath, M. et al. (2009) ‘Pharmacologically induced angiogenesis in transgenic zebrafish’, Biochemical and Biophysical Research Communications, 378(4), pp. 766–771. Available at: https://doi.org/10.1016/j.bbrc.2008.11.127.
M. Raghunath, Y. S. Wong, M. Farooq, and R. Gee, “Pharmacologically induced angiogenesis in transgenic zebrafish,” Biochemical and Biophysical Research Communications, vol. 378, no. 4, pp. 766–771, 2009, doi: 10.1016/j.bbrc.2008.11.127.
RAGHUNATH, Michael, Yuan Sy WONG, Muhammad FAROOQ und Ruowen GEE, 2009. Pharmacologically induced angiogenesis in transgenic zebrafish. Biochemical and Biophysical Research Communications. 2009. Bd. 378, Nr. 4, S. 766–771. DOI 10.1016/j.bbrc.2008.11.127
Raghunath, Michael, Yuan Sy Wong, Muhammad Farooq, and Ruowen Gee. 2009. “Pharmacologically Induced Angiogenesis in Transgenic Zebrafish.” Biochemical and Biophysical Research Communications 378 (4): 766–71. https://doi.org/10.1016/j.bbrc.2008.11.127.
Raghunath, Michael, et al. “Pharmacologically Induced Angiogenesis in Transgenic Zebrafish.” Biochemical and Biophysical Research Communications, vol. 378, no. 4, 2009, pp. 766–71, https://doi.org/10.1016/j.bbrc.2008.11.127.
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