Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation

Rashid, Rafi; Lim, Natalie Sheng Jie; Chee, Stella Min Ling; Png, Si Ning; Wohland, Thorsten; Raghunath, Michael

doi:10.1089/ten.tec.2013.0733

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation
Authors:	Rashid, Rafi Lim, Natalie Sheng Jie Chee, Stella Min Ling Png, Si Ning Wohland, Thorsten Raghunath, Michael
DOI:	10.1089/ten.tec.2013.0733
Published in:	Tissue Engineering - Part C: Methods
Volume(Issue):	20
Issue:	12
Issue Date:	2014
Publisher / Ed. Institution:	Mary Ann Liebert
ISSN:	1937-3392 1937-3384
Language:	English
Subject (DDC):	571: Physiology and related subjects 572: Biochemistry
Abstract:	Macromolecular crowding (MMC) is a biophysical effect that governs biochemical processes inside and outside of cells. Since standard cell culture media lack this effect, the physiological performance of differentiated and progenitor cells, including extracellular matrix (ECM) deposition, is impaired in vitro. To bring back physiological crowdedness to in vitro systems, we have previously introduced carbohydrate-based macromolecules to culture media and have achieved marked improvements with mixed MMC in terms of ECM deposition and differentiation of mesenchymal stem cells (MSCs). We show here that although this system is successful, it is limited, due to viscosity, to only 33% of the fractional volume occupancy (FVO) of full serum, which we calculated to have an FVO of approximately 54% v/v. We show here that full-serum FVO can be achieved using polyvinylpyrrolidone (PVP) 360 kDa. Under these conditions, ECM deposition in human fibroblasts and MSCs is on par, if not stronger than, with original MMC protocols using carbohydrates, but with a viscosity that is not significantly changed. In addition, we have found that the proliferation rate for bone marrow-derived MSCs and fibroblasts increases slightly in the presence of PVP360, similar to that observed with carbohydrate-based crowders. A palette of MMC compounds is now emerging that enables us to tune the crowdedness of culture media seamlessly from interstitial fluid (9% FVO), in which the majority of tissue cells might be based, to serum environments mimicking intravascular conditions. Despite identical FVO's, individual crowder size effects play a role and different cell types appear to have preferences in terms of FVO and the crowder that this is achieved with. However, in the quest of crowders that we have predicted to have a smoother regulatory approval path, PVP is a highly interesting compound, as it has been widely used in the medical and food industries and shows a novel promising use in cell culture and tissue engineering.
URI:	https://digitalcollection.zhaw.ch/handle/11475/12205
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Rashid, R., Lim, N. S. J., Chee, S. M. L., Png, S. N., Wohland, T., & Raghunath, M. (2014). Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation. Tissue Engineering - Part C: Methods, 20(12). https://doi.org/10.1089/ten.tec.2013.0733

Rashid, R. et al. (2014) ‘Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation’, Tissue Engineering - Part C: Methods, 20(12). Available at: https://doi.org/10.1089/ten.tec.2013.0733.

R. Rashid, N. S. J. Lim, S. M. L. Chee, S. N. Png, T. Wohland, and M. Raghunath, “Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation,” Tissue Engineering - Part C: Methods, vol. 20, no. 12, 2014, doi: 10.1089/ten.tec.2013.0733.

RASHID, Rafi, Natalie Sheng Jie LIM, Stella Min Ling CHEE, Si Ning PNG, Thorsten WOHLAND und Michael RAGHUNATH, 2014. Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation. Tissue Engineering - Part C: Methods. 2014. Bd. 20, Nr. 12. DOI 10.1089/ten.tec.2013.0733

Rashid, Rafi, Natalie Sheng Jie Lim, Stella Min Ling Chee, Si Ning Png, Thorsten Wohland, and Michael Raghunath. 2014. “Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation.” Tissue Engineering - Part C: Methods 20 (12). https://doi.org/10.1089/ten.tec.2013.0733.

Rashid, Rafi, et al. “Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation.” Tissue Engineering - Part C: Methods, vol. 20, no. 12, 2014, https://doi.org/10.1089/ten.tec.2013.0733.