RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells

Arai, Satoshi; Kriszt, Rókus; Harada, Kazuki; Looi, Liang‐Sheng; Matsuda, Shogo; Wongso, Devina; Suo, Satoshi; Ishiura, Shoichi; Tseng, Yu‐Hua; Raghunath, Michael; Ito, Toshiro; Tsuboi, Takashi; Kitaguchi, Tetsuya

doi:10.1002/anie.201804304

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells
Authors:	Arai, Satoshi Kriszt, Rókus Harada, Kazuki Looi, Liang‐Sheng Matsuda, Shogo Wongso, Devina Suo, Satoshi Ishiura, Shoichi Tseng, Yu‐Hua Raghunath, Michael Ito, Toshiro Tsuboi, Takashi Kitaguchi, Tetsuya
DOI:	10.1002/anie.201804304
Published in:	Angewandte Chemie: International Edition
Volume(Issue):	57
Issue:	34
Page(s):	10873
Pages to:	10878
Issue Date:	2018
Publisher / Ed. Institution:	Wiley
ISSN:	1433-7851 0570-0833
Language:	English
Subject (DDC):	572: Biochemistry
Abstract:	Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.
URI:	https://digitalcollection.zhaw.ch/handle/11475/12102
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Arai, S., Kriszt, R., Harada, K., Looi, L.-S., Matsuda, S., Wongso, D., Suo, S., Ishiura, S., Tseng, Y.-H., Raghunath, M., Ito, T., Tsuboi, T., & Kitaguchi, T. (2018). RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells. Angewandte Chemie: International Edition, 57(34), 10873–10878. https://doi.org/10.1002/anie.201804304

Arai, S. et al. (2018) ‘RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells’, Angewandte Chemie: International Edition, 57(34), pp. 10873–10878. Available at: https://doi.org/10.1002/anie.201804304.

S. Arai et al., “RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells,” Angewandte Chemie: International Edition, vol. 57, no. 34, pp. 10873–10878, 2018, doi: 10.1002/anie.201804304.

ARAI, Satoshi, Rókus KRISZT, Kazuki HARADA, Liang‐Sheng LOOI, Shogo MATSUDA, Devina WONGSO, Satoshi SUO, Shoichi ISHIURA, Yu‐Hua TSENG, Michael RAGHUNATH, Toshiro ITO, Takashi TSUBOI und Tetsuya KITAGUCHI, 2018. RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells. Angewandte Chemie: International Edition. 2018. Bd. 57, Nr. 34, S. 10873–10878. DOI 10.1002/anie.201804304

Arai, Satoshi, Rókus Kriszt, Kazuki Harada, Liang‐Sheng Looi, Shogo Matsuda, Devina Wongso, Satoshi Suo, et al. 2018. “RGB‐Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells.” Angewandte Chemie: International Edition 57 (34): 10873–78. https://doi.org/10.1002/anie.201804304.

Arai, Satoshi, et al. “RGB‐Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells.” Angewandte Chemie: International Edition, vol. 57, no. 34, 2018, pp. 10873–78, https://doi.org/10.1002/anie.201804304.